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mouse anti dac 1 1  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti dac 1 1
    Mouse Anti Dac 1 1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mabdac1/anti-Dachshund+protein/pmc12962518-444-4-16
    Average 94 stars, based on 9 article reviews
    mouse anti dac 1 1 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: Polycomb-Dependent Chromatin Looping Contributes to Gene Silencing during Drosophila Development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Biotin-14-dATP Life Technologies Cat#19524-016 DNA Polymerase I, Large (Klenow) Fragment NEB Cat#M0210 T4 DNA Ligase NEB Cat#M0202 Proteinase K AMPure XP beads Beckman Coulter Cat#A63881 Qubit dsDNA High Sensitivity Assay Life Technologies Cat#Q32854 Dynabeads MyOne Streptavidin T1 beads Life Technologies Cat#65602 T4 PNK NEB Cat#M0201 T4 DNA polymerase I NEB Cat#M0203 Klenow exo minus NEB Cat#M0212 DNA Quick ligase NEB Cat#M2200 Dpn II NEB Cat#R0543 16% Formaldehyde Solution (w/v), Methanol-free Thermo Fisher Cat#28908 Scientific Complete, EDTA-free, EASYPack Roche Cat#04693132001 Critical Commercial Assays KAPA Library preparation kit Illumina Cat#KK2620 NEXTflex DNA Barcodes - 6 Bioo Scientific Cat#514101 FISH Tag DNA Multicolor Kit, Alexa Fluor dye combination Thermo Fisher Scientific Cat#F32951 Antibody against H3K27me3 ActiveMotif #39155 Antibodies against H3K27ac ActiveMotif #39133 Antibodies against PH, PC, PSC Schuettengruber et al., 2009 Antibody against Dachshund (Dac) Developmental Studies Hybridoma Bank # mAbdac1-1 s Deposited Data In situ Hi-C from hand sorted embryo this paper GEO: GSE103625 ChIP-seq against Pol II in WT and Zld mutant embryo (Sun et al., 2015) GEO: GSE65441 ChIP-seq against histone modification in embryos (Li et al., 2014) GEO:GSE58935 ChIP-seq against Zelda in embryos (Harrison et al., 2011) GEO:GSE30757 ChIP-seq against Gaf and CBP in 2-4h embryos (Koenecke et al., 2017) GEO:GSE68983 ChIP-seq against Ph, Pc (Schuettengruber et al., 2014) GEO:GSE60428 ChIP-seq against histone modification in 14-16hr embryos Modencode consortium ChIP-chip against insulator proteins in 0-12hr embryos Modencode consortium ChIP-seq pol II pSer5 (Blythe and Wieschaus, 2015) GEO:GSE62925 In situ Hi-C from cycle14, zld KD embryos (Hug et al., 2017) ArrayExpress:E-MTAB-4918 RNA-seq data from early embryo (Zenk et al., 2017) ENA: ERP020413 FISH images presented in Figures 2 and 3 Mendeley data https://data.mendeley. com/datasets/k4snfyswr6/2 Experimental Models: Organisms/Strains W1118 W1118 DPRE1 this paper W1118 DPRE2 this paper (Continued on next page) Molecular Cell 71, 73–88.e1–e5, July 5, 2018 e1 MOLCEL 6671

    Immunostaining:

    Article Title: A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer-promoter communication.
    Article Snippet: .. The following primary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: -Dachshund (DAC), 1:400, (DSHB clone number: mAbdac1-1) The following secondary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: - Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 1:1000, (Invitrogen, A-31571) The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for qChIP: -Su(Hw), (1:100) gift from Victor Corces, Antibody is not commercially available The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for CUT&RUN: - H3K27me3, 1:100, Active motif, 39155 - IgG, 1:100, Cell Signaling Technology, 2729S Validation Commercial antibodies are validated for the use of immunofluorescence and CUT&RUN. ..

    Article Title: A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer–promoter communication
    Article Snippet: .. The following primary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: -Dachshund (DAC), 1:400, (DSHB clone number: mAbdac1-1) The following secondary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: - Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 1:1000, (Invitrogen, A-31571) The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for qChIP: -Su(Hw), (1:100) gift from Victor Corces, Antibody is not commercially available The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for CUT&RUN: - H3K27me3, 1:100, Active motif, 39155 - IgG, 1:100, Cell Signaling Technology, 2729S Validation Commercial antibodies are validated for the use of immunofluorescence and CUT&RUN. ..

    Biomarker Discovery:

    Article Title: A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer-promoter communication.
    Article Snippet: .. The following primary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: -Dachshund (DAC), 1:400, (DSHB clone number: mAbdac1-1) The following secondary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: - Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 1:1000, (Invitrogen, A-31571) The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for qChIP: -Su(Hw), (1:100) gift from Victor Corces, Antibody is not commercially available The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for CUT&RUN: - H3K27me3, 1:100, Active motif, 39155 - IgG, 1:100, Cell Signaling Technology, 2729S Validation Commercial antibodies are validated for the use of immunofluorescence and CUT&RUN. ..

    Article Title: A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer–promoter communication
    Article Snippet: .. The following primary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: -Dachshund (DAC), 1:400, (DSHB clone number: mAbdac1-1) The following secondary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: - Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 1:1000, (Invitrogen, A-31571) The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for qChIP: -Su(Hw), (1:100) gift from Victor Corces, Antibody is not commercially available The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for CUT&RUN: - H3K27me3, 1:100, Active motif, 39155 - IgG, 1:100, Cell Signaling Technology, 2729S Validation Commercial antibodies are validated for the use of immunofluorescence and CUT&RUN. ..

    Immunofluorescence:

    Article Title: A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer-promoter communication.
    Article Snippet: .. The following primary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: -Dachshund (DAC), 1:400, (DSHB clone number: mAbdac1-1) The following secondary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: - Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 1:1000, (Invitrogen, A-31571) The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for qChIP: -Su(Hw), (1:100) gift from Victor Corces, Antibody is not commercially available The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for CUT&RUN: - H3K27me3, 1:100, Active motif, 39155 - IgG, 1:100, Cell Signaling Technology, 2729S Validation Commercial antibodies are validated for the use of immunofluorescence and CUT&RUN. ..

    Article Title: A PRE loop at the dac locus acts as a topological chromatin structure that restricts and specifies enhancer–promoter communication
    Article Snippet: .. The following primary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: -Dachshund (DAC), 1:400, (DSHB clone number: mAbdac1-1) The following secondary antibodies were used (Antibody, dilution, Provider, Catalogue number) for immunostaining: - Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 647 1:1000, (Invitrogen, A-31571) The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for qChIP: -Su(Hw), (1:100) gift from Victor Corces, Antibody is not commercially available The following antibodies were used (Antibody, dilution, Provider, Catalogue number) for CUT&RUN: - H3K27me3, 1:100, Active motif, 39155 - IgG, 1:100, Cell Signaling Technology, 2729S Validation Commercial antibodies are validated for the use of immunofluorescence and CUT&RUN. ..



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